In brief, 500 µg isolated mitochondria were resuspended in 100 µl resuspension buffer (250 mM sucrose, 15 mM KH2PO4, 2 mM MgAc2, 0.5 mM EDTA, 0.5 g/l HSA pH 7.2) and stored as 10 µl aliquots at −80 °C ...
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