In brief, 500 µg isolated mitochondria were resuspended in 100 µl resuspension buffer (250 mM sucrose, 15 mM KH2PO4, 2 mM MgAc2, 0.5 mM EDTA, 0.5 g/l HSA pH 7.2) and stored as 10 µl aliquots at −80 °C ...
2021). Briefly, muscle fibers were separated and permeabilized in BIOPS buffer supplemented with saponin (30 µg/mL) and washed in respiration buffer (MiR05; pH 7.0) containing EGTA (0.5 mM), ...
This valuable study identifies a protein called adenosine deaminase-related growth factor (ADGF) as a key regulator of tip formation in the slime mold Dictyostelium discoideum. The authors ...
Diabetes mellitus (DM) is one of the major risk factors for cardiovascular disease (CVD), and CVD is the leading cause of morbidity and mortality worldwide. By 2045, DM is expected to affect 700 ...
Department of Chemistry, Northwestern University, Evanston, Illinois 60208, United States Integrated Molecular Structure Education and Research Center, Northwestern University, Evanston, Illinois ...
KU Leuven, Department of Physics and Astronomy (HF), Celestijnenlaan 200D, Leuven 3001, Belgium ...
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