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Formalin-fixation causes fragmentation and chemical modifications in DNA, posing significant challenges for molecular assays, such as downstream NGS applications. The DEPArrayâ„¢ FFPE QC Kit has ...
Sperm DNA fragmentation was evaluated using the TUNEL assay (Apo-Direct kit; Pharmingen, San Diego, CA), followed by flow cytometry analysis. A cut-off value of 19.25% fragmentation maximized the ...
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