Because the egg cell is only 100 micrometers, or one-tenth of a millimeter, wide, we monitor this fine surgical extraction with a microscope (see previous image ... stem cells grown in culture ...
Centrifuge the cells at 200g for 5 min at 15–25 °C and resuspend the pellet in 10 ml MEF medium supplemented with 4 ng/ml bFGF. To prepare the appropriate culture vessels for plating the ...
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