pET-28a(+) Sequence and Map - SnapGene
WEBBacterial vector for expression of N-terminally 6xHis-tagged proteins with a thrombin site. For other reading frames, use pET-28b(+) or pET-28c(+).
Addgene: Vector Database - pET-28 a (+)
WEBpET-28 a (+) Vector Database. Welcome to Vector Database! Vector database is a digital-only collection of vector backbone information compiled by Addgene from third party sources. This vector is NOT available from Addgene and the database is no longer actively maintained. Search vector database. This vector is not available from Addgene.
Improved designs for pET expression plasmids increase protein ...
WEBMay 7, 2020 · pET28a is the most popular expression plasmid on the market (described in >40,000 published articles). It contains the T7 promoter and an adjacent lac operator sequence that is included to...
pET-28a(+) vector map and sequence - novoprolabs.com
WEBpET-28a(+) is a bacterial expression vector designed for the production of N-terminally 6xHis-tagged proteins with a thrombin cleavage site. This vector is particularly suitable for proteins that require post-translational processing to remove the His-tag.
pET-28a(+) - EcoliWiki
WEBAug 16, 2011 · The pET-28a(+) vector carries an N-terminal His•Tag/thrombin/T7•Tag configuration in addition to an optional C-terminal His•Tag sequence. Unique sites are shown on the circle map. The T7 expression region is reversed on the circular map due to the sequence numbering from the pBR322 convention.
Improved designs for pET expression plasmids increase protein ...
WEBMay 7, 2020 · pET28a is the most popular expression plasmid on the market (described in >40,000 published articles). It contains the T7 promoter and an adjacent lac operator sequence that is included to suppress uninduced expression 5.
Design of improved pET expression plasmids | Nature Methods
WEBJul 2, 2020 · Shilling et al. have revisited the design of the pET28a plasmid, the most popular of the series. They first added the full T7 promoter sequence, which had originally been truncated upon insertion...
Rapid modification of the pET-28 expression vector for ligation ...
WEBThe pET-28b(+) expression vector used as the template for plasmid modification was obtained from Novagen. S. cerevisiae strain BJ5464-NpgA (MATα ura3-52 his3-Δ200 leu2-Δ1 trp1 pep4∷HIS3 prb1 Δ1.6R can1 GAL) was used for the recombinatorial assembly of PCR products (Ma et al., 2009).
pET-28a(+) DNA, N-terminal His-Tag/thrombin/T7-Tag Sigma-Aldrich
WEBpET-28a(+) DNA - Novagen Novagen′s pET-28a-c(+) vectors carry an N-terminal His•Tag/thrombin/T7•Tag configuration plus an optional C-terminal His•Tag sequence.; find -69864 MSDS, related peer-reviewed papers, technical documents, similar products & more at Sigma-Aldrich
DNASU Plasmid | Detailed Vector Information: pET28a
WEBVector Name: pET28a. Synonyms: pET-28a, pET28. Sequencing Primer: Forward: T7. Reverse: T7 terminator. Description: Bacterial expression vector with T7lac promoter, adds N-terminal His tag, thrombin cleavage site, internal T7 epitope tag, C-terminal His tag; kanamycin resistance; restriction enzyme cloning.
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